Leica TCS SP8
An spectral upright confocal microscope system with white light laser (WLL) with up to eight excitation lines which can be used simultaneously. By tuning both excitation and detection, complete excitation emission spectra can be acquired. With this spectral information, any dye can be optimally excited with minimum cross-excitation and specimen damage.
Prostaglandin-endoperoxide synthase 2 (PTGS2) expression in a newly formed corpus luteum in a mouse ovary. The image was taken by confocal microscopy. Image courtesy of Noura Massri, Dr. Ripla Arora’s Lab. A mouse uterus with an embryo implantation site during early pregnancy is shown. The embryo forms a V-shaped implantation chamber and the blood vessels remodel around the implantation chamber. Purple indicates luminal and glandular epithelium (branched structures) and pink indicates vasculature. The image was taken using a confocal microscope (Leica SP8X) at 10x magnification. Image courtesy of Dr. Manoj Madhavan, Dr. Ripla Arora’s Lab. An image of a longitudinal mouse uterine cross section where the uterine lumen, glands, and stroma are visible was captured using confocal microscopy. The tissue was stained to identify E-cadherin (green) on the membrane of epithelial cells and progesterone receptor (red), which is expressed nuclearly in the luminal epithelium, glandular epithelium, stroma, and muscle. This tissue section is from a mouse at gestational day 3.5 of pregnancy, a critical stage right before embryos implant into the uterine lining. Image courtesy of Katrina Granger, Dr. Ripla Arora’s Lab.