Leica TCS SP8 X
Leica TCS SP8 multi-photon (MP) system is equipped with white light laser spectral confocal and multi-photon system with insight X3 dual laser. It makes use of long wavelengths of fully integrated infrared (IR) excitation lasers, the high efficiency of hybrid detectors (HyD), and an infrared-optimized optical transmission. These allow to deeply penetrate into the tissue with super-sensitivity and to uncover the finest details of cellular and subcellular processes.
Top view of a 1mm full-thickness wound four weeks after induction shows how lymphatic vessels regenerate during wound healing in mice skin. Red= Langerhans cells (huLangerin-CreER;Rosa-stop-tdTomato); green= lymphatic endothelial cells (Prox1-EGFP); white= nuclei of epidermal epithelial cells (K14-H2B-Cerulean); magenta= collagen (Second Harmonic Generation). Image acquired in vivo using a Leica SP8 DIVE multiphoton microscope. Image courtesy of Axel Schmitter, Dr. Sangbum Park’s Lab. 
“Skin Corals” In a two-photon photograph of a mouse ear epidermis, Langerhans cells (green), keratinocytes (red), and a layer of connective tissue separating the dermis and epidermis (blue) can be observed. Image courtesy of Dr. Artem Kiselev, Dr. Sangbum Park’s Lab. 
Skin helix: Cryosection view of Mouse-ear skin. The red color is nuclei of epidermal epithelial cells (K14-H2B-mCherry), whereas the collagen looks blue (second harmonic generation). The Images were acquired in vivo using a Leica SP8 DIVE multiphoton microscope. Image courtesy of Sudhanshu Mishra, Dr. Sangbum Park’s Lab.